Relationship Between the Types of the Extraction Buffer with Quality of the 2-Dimensional Electrophoresis Proteomic Map

Objective: It seems that the type of buffer for extraction of proteins from each sample is important. However, the most widely used buffer in these researches is rehydration buffer. In this study, we compared the 2 dimensional electrophoresis (2-DE) maps from different buffers. Materials and methods: 2-DE map of rat testicular tissue obtained by three distinct buffer included phosphate buffer saline (PBS (A), Tris-HCl (B), or lysis buffer (C) were evaluated and compared. Results: 2-DE map of rat testicular tissue obtained by three distinct buffer included phosphate buffer saline (PBS (A), Tris-HCl (B), or lysis buffer (C) were evaluated and compared. Conclusion: Our results demonstrated that using buffer 0.05 M Tris-HCl, 10% sucrose, pH 6.80 was provided good comparable results with rehydration buffer. However, buffer A was not appropriate for 2-DE. Due to the expensiveness of CHAPS and ampholite, which were used in the rehydration buffer, the replacing of it with buffer B that produced good 2-DE is recommended.