COMPARISON OF CELISA AND BLOOD SMEARS TO ASSESS ANAPLASMA SPP INCIDENCE IN DWARF GOATS FROM BEOUMI (COTE D?IVOIRE)

A study of dwarf goats in B?oumi (C?te d'Ivoire) aimed to compare two diagnostic techniques in the fight against Anaplasma spp. The competitive immunoenzymatic technique (cELISA) and technique of direct observation of blood smears with optical microscope. A total of 540 blood samples were taken from 270 apparently healthy dwarf goats and analysed. Method consisted firstly of collecting 270 blood samples from the jugular vein and of looking for anti-Anaplasma antibodies using the cELISA test. Then, 270 blood smears were made from blood taken at the level of the auricular vein of these same animals to also detect Anaplasmaspp with optical microscope. Results obtained showed that 56 sera were positive to anti-Anaplasma antibodies with a seroprevalence of 20.74%. Similarly, observation of the smears with microscope showed that 12 slides were infested by Anaplasmaspp with prevalence of 4.44%. Significant difference was observed between the two prevalence obtained according chi-square test. Moreover, the comparison between the two tests used showed a low agreement. However, AnaplasmamarginaleandAnaplasmacentrale two species of the genus Anaplasma were identified in this study. These results will help select appropriate diagnostic methods to establish an adequate strategy for the control of Anaplasma spp.