The Experimental Wound Healing using Coffee Powder and Honey Compared to NPWT

Today the Negative Pressure Therapy (NPWT) applied to treat wounds. The procedure needs to criticize because not simple, expensive, use only by doctors, and limited availability. The wound treatment using Robusta coffee powder compared to honey, NPWT, and control groups. The wound sized 2cmx2cmx0.20 cm made at the back skin. Each wound infected by 1 ml. of a bacterial suspension of Staphylococcus aureus ATCC 25923. The grade of epithelialization, angiogenesis, collagenization, on the 7th day, 14th day, were observed. The randomized observational cohort analytic research conducted in 250 gram-Wistar rats divided into three groups, each group of 5 rats. On the 7thday and the14th-day, the wound biopsied and put into formalin solution. The epithelial cell growth inspected using 200 x-magnification. The healing parameter using the grade of epithelialization 3=normal, grade 2=low epithelization, 1=no growth; grade of angiogenesis 3=more than two new blood vessels; 2=one to two blood vessels; 1=no new blood vessel; grade of collagenization 3=more than normal collagen density; 2=the same with normal collagen density; 1=low than normal collagen density. Data analysis using the Kruskal-Wallis test. Statistics analyzed using SPSS 24.0; p-value < 0.05 considered significant. The grade of epithelialization, angiogenesis, collagenization were not statistically significant in the 7th day groups of control, Robusta coffee, honey, NPWT, with p=1.000. In the 14th day groups of control, Robusta coffee, honey, NPWT, the epithelialization, and angiogenesis have p=1.000, and the collagenization on the 14th day has p=0.271. CONCLUSIONS The wound healing using coffee powder or honey was not different compared with NPWT.