EFFICACY OF PLATELET RICH FIBRIN IN COMBINATION WITH ENAMEL MATRIX DERIVATIVE ON PERIODONTAL TISSUES REGENERATION IN RATS

Objectives: Evaluating the efficacy of enamel matrix derivative in combination with platelet rich fibrin on periodontal tissues regeneration in rats. Material and methods: This experimental trail that was conducted on twenty-four adult healthy male Wistsrrats, animals were housed in separated cages as two rats per cage. Rats were kept in suitable conditions like temperature, humidity, standard food for rodents. Animals randomly allocated into 3 groups (8 rats each); G1: control group, left to heal without intervention, G2: treated by enamel matrix derivatives (EMD), G3: treated by enamel matrix derivatives plus platelet rich fibrin (EMD+PRF). Induced periodontal fenestration defects were created at buccal side of the distal root of the 1st mandibular molar and the mesial root of the 2ed mandibular molar. After surgery rats housed in a clean sterile cage and received special care. After induction of the fenestration defect by one month, four rats from each group were sacrificed. While, the remaining four rats of each group were sacrificed after another one month. Results: Histological analysis showed that periodontal tissues had best regeneration of periodontal fibers with more mature bone formation in the EMD+PRF group (G3) comparing to other groups. In addition, other groups showed favorable regeneration of periodontal tissues. Conclusions: Application platelet rich fibrin in combination with biological mediators such as enamel matrix protein in periodontal treatment improves regeneration of periodontal tissues.