NANOSTRUCTURED FERRIC CITRATE EFFECT ON Chlorella vulgaris DEVELOPMENT
Journal: Biotechnologia Acta (Vol.11, No. 6)Publication Date: 2018-12-30
Authors : N. B. Golub M. Tsvetkovych I. I. Levtun V. I. Maksyn;
Page : 47-54
Keywords : chlorella cells; biomass; intracellular iron; chlorophylls; carotenoids; nanostructured ferric citrate.;
Abstract
The aim of the research was to study the development of Chlorella vulgaris at culturing on the modified Gromov 6 medium with high concentrations of nanostructured ferric citrate and also its effect on photosynthesis pigments accumulation. It was demonstrated that the highest intracellular iron content (15 mg/g of dry mass) in the culture cells was typical with nanostructured ferric citrate content of 30 mg/dm3 of culture medium, the highest content of chlorophyll a — 23 mg/g of dry algae mass, b — 7.5 mg/g of dry mass, and carotenoids — 9.2 mg/g of dry mass was observed at nanostructured ferric citrate content of 20 mg/dm3. The use of nanostructured ferric citrate leaded to an increase in the chlorella biomass yield by 3 times with compared to standard technology. Simultaneously, intracellular iron content in cells increased significantly with the use of nanostructured ferric citrate, which increases their value as a nutritional supplement. In order to increase the biomass yield and intracellular iron content in cells, application of nanostructured ferric citrate is recommended.
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