Relative Assessment of Some Toxins on Molecular Changes in the Protein Profile of Mugil Cephalus, Peaneaus Indicus and Protues Pelagicus Procured from some Estuaries of South East Coast of Indian Ocean
Journal: International Journal of Oceanography & Aquaculture (Vol.2, No. 6)Publication Date: 2018-12-06
Authors : Hassan Sulieman MA; El Amin S;
Page : 1-9
Keywords : Heavy Metals; Total Protein; Mullet; Shrimp; Crab;
Abstract
Marine organisms namely Mugil cephalus, Portunus pelagicus, and Penaeus indicus were collected during post-monsoon, pre-monsoon and monsoon from Vellar and Uppanar estuaries, South East Coast of Indian Ocean. Muscle of considered species was examined for a presence of toxic metals (Al, Cd, Cu, Pb and Zn), utilizing Inductively Coupled Optical Emission Spectrometry (ICP-OES). The outcome demonstrated the Aluminum concentration (12.176µg/g) had a most extreme level in considered marine organisms at Uppanar estuary took after by zinc (1.332 µg/g), copper (0.204 µg/g), lead (0.054 µg/g) and cadmium (0.004µg/g) when contrasted and Vellar estuary Al (2.084µg/g), Zn (0.307µg/g), Cu (0.145 µg/g), Pb (0.054µg/g) and Cd (0.026µg/g). None of the toxic metals examined at Vellar and Uppanar estuaries was exceeding acceptable levels. The impact of these trace metals on protein were investigated quantitatively and subjectively. Likewise, utilizing sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and total protein estimation. The most level of total protein was found in Mugil cephalus muscle of (381.8mg/ml) in monsoon and Penaeus indicus (377.0mg/ml) in pre-monsoon at Vellar estuary and the base level was found in Mugil cephalus (211.8mg/ml) at Uppanar estuary in the same season. Total protein level was watched lower in all test species of Uppanar estuary than the species of Vellar estuary. SDS-PAGE examination as protein profile demonstrating the little varieties in protein divisions in correlation among species gathered from two localities.
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