The Change of the Ceruloplasmin Level in Blood Plasma in Conditions of Myoglobinuric Acute Kidney Injury

Acute kidney injury is a common problem in a clinic of critical emergency medicine, which is usually accompanied by high mortality. Ceruloplasmin is a plasma antioxidant that prevents the formation of toxic iron ion, activation of lipid peroxidation and protects the structure of lipid membranes from the damage. This enzyme is also involved in the detoxification of superoxide anions in the extracellular medium. The aim of the research is to study the changes in the levels of ceruloplasmin in blood plasma and product of lipid peroxidation malonic aldehyde (MA) in blood and kidney tissue under the conditions of mioglobinuric acute kidney injury on the background of ceruloplasmin administration. Materials and methods. The experimental studies were performed on white nonlinear rats. We modeled the acute kidney injury by intramuscular administration of 50% glycerol solution at a dose of 8 mg/kg. Ceruloplasmin was administered intraperitoneally at a dose of 7 mg/kg/day. The level of ceruloplasmin in blood plasma and the level of malonic aldehyde in blood and kidney tissue was determined 24 h, 48 h, and 72 h after glycerol administration and induction of acute kidney injury. Results and discussion. The performed experimental studies indicated a significant deterioration of the prooxidant-antioxidant balance in the blood and of kidney tissue under the conditions of acute kidney injury. At the same time, there was a decrease in antioxidant defense. The ceruloplasmin administration to rats with acute kidney injury leads to decrease in the intensity of lipoperoxidation processes, as evidenced by the parameters of malonic aldehyde in the tissue of the kidneys and blood compared to animals with a model pathological process. At the same time, an increase in the level of ceruloplasmin in blood plasma improves antioxidant defense under the condition of acute kidney injury. Conclusion. The research showed that ceruloplasmin administration in acute kidney injury decreased the intensity of free radical reactions in kidney tissue and blood and improved antioxidant protection by reducing lipid peroxidation. It also prevented the further development of destructive processes in kidneys.