AEROBIC BACTERIOLOGICAL PROFILE OF SURGICAL SITE INFECTIONS WITH ANTIBIOGRAM

The present study was done on 236 clinically suspected pus samples from patients who underwent surgery between Jan 2017 to June 2017 from Obstetrics and gynecology , General surgery and Orthopedic departments in Government General Hospital,Vijayawada.This study was done to know the incidence of surgical site infections and the prevalence of aerobic bacterial pathogens along with their antibiogram. Surgical site infections (SSI) are the second most common nosocomial infections and have adverse impact on patients. Isolation and identification of the organisms was done by gram stains and culture growth. Antimicrobial susceptibility testing was performed by Kirby- Bauer disc diffusion method and results were interpreted as per Clinical and Laboratory Standards Institute(CLSI) guidelines. Out of 236 clinically suspected pus samples received in the microbiology laboratory, Siddhartha Medical College, 137 (58%) were culture positive and 99(41.9%) were culture negative. The infection was found to be higher in female patients in age group 21-30yrs than in male patients. The most commonly isolated pathogens were Staphylococcus aureus 38(27.7%) followed by Klebsiella species 29(21.1%)and Escherichia coli 26(18.9%). Antimicrobial profile of gram positive isolates revealed highest sensitivity to amikacin (68.4%),followed by linezolid(60.5%),whereas gram negative isolates showed most sensitivity to amikacin (58.6%) and piperacillin tazobactum (44.8%).SSI are responsible for increasing the treatment cost, length of hospital stay and significant morbidity and mortality.Hence proper, formulation of infection control policies, hand hygiene techniques and optimal preoperative, intraoperative and postoperative patient care can be implemented to reduce the incidence of surgical site infections.