Topical Preparation and Antimicrobial Study of Crude Extracts of Syzygium cumini (L) Skeel Leaves

The aim of the study is antimicrobial study of crude extracts of S. cumini leaves and further topical preparation from Methanolic extract. For this successive soxhlet extracted petroleum ether, diethyl ether, methanol and aqueous extracts of leaves of S. cumini were subjected to antimicrobial activity test (antibacterial and anti-fungal test). Antimicrobial activity testing was done by agar well diffusion method. The organism tested were gram positive organism: Staphylococcus aureus (ATCC) and MRSA and gram negative organism: E. coli, Pseudomonas aeruginosa, Klebsellapneumonia for antibacterial and candida albican for antifungal Excellent antimicrobial activity was shown by Methanolic extract in comparison to other extract. From the result of antibacterial test of plant extract further gel and ointment were formulated from Methanolic extract which too showed antibacterial activity comparable to plant extract.