Analysis of DNA Content of Wild and Cultured Labeo Calbasu Hamilton, 1822 Using Nanophotometer

Labeo calbasu is an important food fish and commonly known as the "calbasu" or "Black Rohu". The present study was conducted on DNA extraction and determination of DNA quantity of both male and female of wild and cultured Labeo calbasu using Nanophotometer. DNA isolation was done and gel electrophoresis was carried out. Extracted DNA was analyzed using nanophotometer Nanophotometer P330 Implen, Germany to determine the concentration of DNA and its purity level. Several different methods have been employed in the quantification of nuclear DNA over the past 50 years. The ease of use of this technique not only makes it a feasible option for small volume analysis of DNA but also a practical alternative for spectrophotometric measurement. Significant progress was made to measure micro volume liquid samples 1µl in biotechnology and pharmaceutical applications. For the present study both male and female of wild Labeo calbasu from middle Ganga Region and cultured Labeo calbasu from culture ponds and hatchery were taken. The value of DNA concentration in female of wild Labeo calbasu was in between 58 -62 ng µl and of male was in between range of 64- 68 ng µl. The value of DNA concentration in female of cultured Labeo calbasu was 60- 66 ng µl and of male 68- 74 ng µl. Nisha Rana | Seema Jain "Analysis of DNA Content of Wild and Cultured Labeo Calbasu (Hamilton, 1822) Using Nanophotometer" Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-3 | Issue-1 , December 2018, URL: http://www.ijtsrd.com/papers/ijtsrd19105.pdf http://www.ijtsrd.com/biological-science/zoology/19105/analysis-of-dna-content-of-wild-and-cultured-labeo-calbasu-hamilton-1822-using-nanophotometer/nisha-rana