Biofilm formation capability of bacteria, a threat to treat multi drug resistant diabetic foot infection isolates

Bacterial biofilms are complex structured communities surrounded in a matrix of extra-cellular polymeric substances growing on rough surfaces. It is involved in multiple functions like trapping of nutrients, adherence, stability of biofilm structure and the penetration of many antibiotics.250Pus samples were collected from the depth of ulcers from each patient using Amies Transport Swabs. The specimens were inoculated on appropriate media. Biochemical characteristics of pathogens were observed by performing conventional biochemical tests and Quick Test Strips. Antibiotic resistance of isolates was checked by Kirby Bauer's Disc Diffusion Method. Biofilm formation was initially detected by Congo Red Agar Method. Major biofilm forming isolates from different hospitals environment were Klebsiella spp., Corynebacterium spp., S. epidermidis while from hospital curtain's samples, Shigella dysenteriae, Candida albicans and Aspergillus flavus were having capability of biofilm formation. S. aureus showed good biofilm forming ability in presence of 0.2% glucose, sucrose and lactose, P. aeruginosa showed enhancement in biofilm formation in presence of sucrose, glucose, and maltose while E. coli indicated increased biofilm formation in presence of lactose, sucrose and glucose. Most of the biofilm forming isolates were Multi drug resistant which indicate that extracellular material released by these pathogens cover the bacteria cell and hinder the antibiotic