The Effect of Seed-Priming by Cobalt-Diglycyrrhizinate on Wheat (Triticum Aestivum L.) Genome

The aim of our study was to monitor the genomic changes of wheat (Triticum aestivum L.) during seed priming with natural elicitor cobalt-diglycyrrhizinate by RAPD markers. The genomic response to wheat seed priming was analysed with respect to the elicitor concentration (four different concentrations), the type of primer used (six random primers) and the type of responsive tissue (roots and leaves of in vitro seedlings). The level of genome response to seed priming was statistically dependent on elicitor concentration (P<0.001). The type of used primer has proven to have an amplification effect (P<0.001) on generating amplicons in the context of elicitor concentration. The genomic response to seed priming was tissue-specific. The priming effect was observed to a greater extent in leaves than in roots (P<0.001). Our results proved the impact of seed priming by natural elicitor, cobalt-diglycyrrhizinate on wheat genome polymorphism