Estimation of Carbamazepine in Bulk and Pharmaceutical Dosage Form By Zero Order and Area under the Curve UV Spectrophotometric Methods

The aim of the present research was broadly focused on the estimation of carbamazepine in bulk and pharmaceutical dosage form by using two UV –Spectrophotometric methods namely, Zero order UV spectrophotometry (Method -1) and Area under the curve UV spectrophotometry (Method -2). The Zero order UV Spectrophotometric method was based on the measurement drug absorbance at wave length of 284 nm, which was its wavelength of maximum absorbance .The Area under the curve method, was based on the calculation of area occupied by the UV absorbance curve between 278-290 nm. The solvent employed for both methods was 50% v/v ethanol. In the estimation of Carbamazepine, both the methods showed linearity in the range of 2-12µg/ml. The correlation co-efficient was ≥ 0.999. The precision for both the methods was ≤2% RSD. The accuracy was performed by using percentage recovery studies of standard drug spiked at 50,100 and 150% of the test concentration and the values obtained were within the limits. The developed methods were applied for the assay of the drug in its respective dosage forms. The assay of pharmaceuticals dosage form was found to be within limits. All the results were satisfactory, the developed methods can be routinely used for the analysis of the drugs in both bulk and dosage forms.