Immunocytochemical Reactions in the Brittle-Star: Ophiocomina Nigra after Immunization

Immunizations of Ophiocomina nigra were performed with either Bovine Serum Albumine (BSA) or Horse-Radish Peroxydase (HRP) in aquarium containing running sea water. An immunocytochemical test was done in presence of HRP, diaminobenzidin, H2O2 for both animals: Control ones, BSA ones and HRP ones. Positive reactions only occur in injected animals to HRP. So the brittle star O.nigra as the sea stars: Asterina gibbosa discriminates HRP from BSA: it is an antibody-like reaction. Plasmolymphocytic cells were evoked.