DESIGN AND DEVELOPMENT OF THERMAL CYCLER (PCR INSTRUMENT) FOR DNA ANALYSIS
Journal: International Journal of Engineering Sciences & Research Technology (IJESRT) (Vol.8, No. 5)Publication Date: 2019-05-30
Authors : Sailaja Vangimalla Ramesh Datla Lakshmi Prasad M Nagabhushan Raju K;
Page : 80-86
Keywords : Thermal Cycler; Polymerase Chain Reaction; PID Controller; Tuning.;
Abstract
In this work, we designed and developed a rapid Thermal Cycler with thermoelectric cooling for the fast thermo cycling process. The thermal cycler is an instrument that employs precise temperature control and rapid temperature changes to conduct the polymerase chain reaction (PCR). The developed PCR system utilizes low power commercial thermoelectric module which allows fast heating and rapid cooling in the PCR temperature cycles. The experimental results of developed system show that efficiency of Thermal cycling of aluminum block. Unsuitable temperature controller setting will cause the block temperature overshooting or undershooting of the desired block's temperature which can result in damage to the DNA sample and cause incomplete reactions. The effect of Proportional Integral (PI) temperature controller on the performance of thermal cycler is investigated in the present study.
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