FINGER PRINTING ANALYSIS OF THE PHENOLS FROM PERGULARIA DAEMIA FORSK PLANT LEAVES and stem USING HPTLC

The research study was carried out to identify the phenols from methanol extracts (MPD) of medicinally and efficiently useful leaves and stem of Pergularia daemia (Forsk) plant using High Performance Thin Layer Chromatography (HPTLC) technique. Preliminary phytochemical screening was done and HPTLC studies were carried out. Densitometry scanning was performed in the reflectance absorbance mode at 540 nm and operated by Win CATS software with the help of tungsten lamp. Preliminary phytochemical screening of methanol extract of P. daemia shows the presence of alkaloids, glycosides, flavonoids, phenols and carbohydrates. HPTLC finger printing of phenols of methanolic extract of leaf and stem revealed eleven polyvalent phytoconstituents (13 and 10 peaks) and corresponding ascending order of Rf values in the range from 0.08 to 0.65 in leaf and 0.02 to 0.66 in the stem. From the result of preliminary phytochemical analysis and above Rf values from HPTLC, we have concluded the presence of phenols in methanol extracts.