Assay Of Detoxification Potential Of DL-Methionine On Dietary Gliricidia Leaf Meal In Rabbit Nutrition Relative Organ Weight And Blood Indices

An investigation on the detoxification potential of DL-Methionine on Gliricidia Leaf Meal GLM was performed. Gliricidia sepium leaves were dried and milled into meal GLM and incorporated in diets at 0 F0 control 10 F10 and 15 F15 raw and at 10 FSM10 and 15 FSM15 with 0.3 DL-methionine supplementation. 60-weaned rabbits of 49-days old averaging 106616.3 grabbit were used as test animal models and fed the 5-diets to appetite over a feeding trial lasting 42-days. At the age of 912 days 4-rabbits were sampled per treatment for internal organ weight and three 3 for haematological and biochemical studies. Dietary treatment gave no significant differences p0.05 for all the relative organ weights liver kidneys heart and spleen but the lungs p0.05. Haematological parameters such as PCV RBC as well as MCH MCV and MCHC were not significantly affected by the dietary treatment P0.05 while WBC counts decreased in diets FSM10 F15 and FSM15. The biochemical determinants glucose albumin globulin ALT ALP Na P Mg2Cl- HCO3- measured on the rabbits fed the test diets were comparable to those fed the conventional diets p0.05. Diet F15 significantly increased AST enzyme p0.05 while ALT value was significantly augmented p0.05 in all the experimental treatments compared to the control diet. Besides methionine supplementation in diet FSM10 and FSM15 reduced ALT activities compared to methionine unsupplemented diets F10 and F15 respectively. However this latter was not statistically significant p0.05. Dietary leaf meal of G. sepium with or without methionine treatment had no significant effect p0.05 on blood concentration of sodium Na phosphorus P magnesium Mg2 chloride and bicarbonate ions HCO3-. Calcium electrolyte was increased p0.05 with dietary treatments compared to the conventional diet F0. In summary GLM at level up to15 in diets had no adverse effects on internal organs status haematological parameters some biochemical determinants and electrolyte profile of growing rabbits. Elevation in serum ALT and AST suggested that antinutrients in GLM elicited mild tissue lesions in rabbit which is slightly corrected by methionine supplementation. It is recommended that in subsequent researches methionine supplementation to facilitate detoxification should include other detoxicant like Choline chloride and Polyethylene Glycol PEG.