Abortive and Teratogenicity of Ethyl Acetate Extract Fraction of Eugenia Jambolana Seed in Pregnant Rats

The present study was aimed to explore the post-coital contraceptive activity and Teratogenicity effect of ethyl acetate extract fraction of Eugenia Jambolana seed in pregnant rats. In the previous experiments the ethyl acetate extract of Eugenia Jambolana seeds exhibited promising anti-implantation and antiestrogenic activity in female albino rats was examined for the isolation of its active fractions. Two fractions (I and II) were obtained using Thin Layer Chromatography (TLC) of the extract. Both fractions were subjected for testing post-coital contraceptive activity and Teratogenicity effect in pregnant rats. The fractions were administered orally at the dose level of 100 and 300mg/kg body weight from 10 to 18 days of pregnancy. The fraction I of ethyl acetate seed extract treated rats has showed strong abortifacient activity (100%) at 300mg/kg body weight, but did not show any developmental toxicity and Teratogenicity effect in treated rats. Phytochemical studies of fraction I of ethyl acetate extract revealed the presence of flavonoids and a mixture of compounds. These results suggest that a chromatographic fraction I of ethyl acetate extract of Eugenia Jambolana might be used as a contraceptive in the females