Multivariate curve resolution-alternating least squares applied to kinetic spectrophotometric data for the determination of mixtures of aliphatic amines

Kinetic spectrophotometric second order data based on the reaction of 1,2-naphthoquinone-4-sulphonate (NQS) coupled with multivariate curve resolution-alternating least squares (MCR-ALS) has been proposed for simultaneous determination of ethylamine, propylamine and butylamine. Using second-order advantage, MCR-ALS methodology can solve problems of quantitation of analyte in the presence of unknown and uncalibrated interferences. Ethylamine, propylamine and butylamine react differentially with NQS at pH 9.5. Therefore, determination of these amines has been carried out due to the difference between their reaction rates. Quantitative determination of each amine in the mixture is performed using a synthetic solution as standard containing only the amine of interest. The MCR-ALS results are evaluated by the residuals and parameters such as lack of fit. The quantitative determination of these amines in different synthetic mixtures and some real samples such as river water, well water, tap water and soil has been performed and the results have been found to have good recoveries.