Polymorphism of 5′ Flanking Region of Lactoferrin Gene in Khuzestan Buffaloes
Journal: International Journal of Advanced Biological and Biomedical Research (Vol.1, No. 7)Publication Date: 2013-07-01
Authors : Maryam Khatibi; Hedayatullah Roshanfekr; jamal Fayazi; Khalil Mirzade;
Page : 777-782
Keywords : Khuzestan buffalo; Lactoferrin gene; Polymorphism; PCR-SSCP;
Abstract
Lactoferrin is a glycoprotein with molecular weight 80 kDa iron-binding bond, which is composed of 690 amino acids. In most mammalian body fluids such as sweat, semen, tears, and saliva and milk neutrophil granules there. bovine lactoferrin gene be associated with susceptibility/resistance to mastitis and even with some economically important production traits. This study was carried out to detect polymorphism in lactoferrin gene in 85 Bufallo to 4 location khuzestan province were selected randomly. After extracting DNA, amplification of 367 bp of lactoferrin gene was performed using specific primers. In this research four SSCP pattern for AA, BB, EE and FF lactoferrin gene in Bufallo were found in Khuzestan Province. The results showed that lactoferrin gene is so polymorphism in studied population. Gene frequencies for A, B, E and F alleles were 0.341, 0.259, 0.118 and 0.282 respectively. Therefore, the results confirmed that SSCP technique can be used to identify different genotypic variation in these breeds and is a useful tool for selection programs based on marker-assisted selection
Other Latest Articles
- Compression of Breast Cancer Images By Principal Component Analysis
- The Effect of Water Quality and Irrigation Methods on Moisture and Salinity Distribution of Soil
- Analysis of Heart Rate Variability During Meditative and Non-Meditative State Using Analysis of Variance
- Contraceptive Efficacy of Hydro-Methanolic Fruit Extract of Xylopia Aethiopica in Male Albino Rats
- Estimation of Combining Ability and Gene Action for Agro-Morphological Characters of Rapeseed (Brassica Napus L.) Using Line×Tester Mating Design
Last modified: 2019-07-05 02:37:11