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Evaluation of Leaf Protein Pattern in Wheat Genotypes Under Drought Stress

Journal: International Journal of Advanced Biological and Biomedical Research (Vol.2, No. 3)

Publication Date:

Authors : ; ; ; ;

Page : 840-846

Keywords : Drought stress; electrophoresis; Protein pattern; Wheat;

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Abstract

Drought is one of the most important factors limiting crop yields around the world. Drought stress in plants, the change (increase or decrease) in the production of plant proteins. This research was carried out using bread wheat genotypes. For evaluation of leaf protein pattern in wheat, 10 genotypes were assayed with 3 replications under irrigated (non-stress) and rain-fed (stress) conditions. At grain filling stage, 10 random plants were selected and flag leaf samples were harvested. SDS-PAGE Electrophoresis was used to evaluate protein pattern after applying water stress. Thirty five protein bands appeared. Most of the bands were similar in the entries and specific bands were rare. Under drought stress, high molecular weight proteins were intensified, while low molecular weight proteins were faint. Cluster analysis under non-stress conditions classified the genotypes into tree clusters but under stress conditions the entries were classified into four clusters.

Last modified: 2019-07-05 03:01:54