Statistical Optimization of Conditions for Maximize Production of Mannan by Saccharomyces Cerevisiae Using Response Surface Methodology
Journal: International Journal of Advanced Biological and Biomedical Research (Vol.2, No. 42)Publication Date: 2014-04-15
Authors : Hanneh vakilian Aghooi; Seyed Ali Mortazavi; Elnaz Milani; Arash koochaki; Masoome Mehraban;
Page : 974-984
Keywords : Statistical Optimization; Saccharomyces cerevisiae;
Abstract
In view of the increase in Saccharomyces cerevisiae mannan content, the culture condition for S.cerevisiae were optimized in this study. The influence of culture condition such as original pH, inoculum size, and temperature on mannan production were evaluated using Response surface methodology. The mathematical model was established by the quadratic rotary combination design. with the order of effect as follows: temperature > initial pH > inoculation size. The optimized culture condition was pH, 6.6; inoculum size, 4 ml; temperature, 32°C. The maximum mannan production increased to 94.912 ±9.1 mg/100 mL at the optimum culture condition.It was evident that the mannan production was affected significantly by culture condition optimization (p < 0.01). After obtaining the optimal conditions, modified alkaline method was used to purify the extracted Mannan. FT-IR spectra analysis showed that the product was chemically pure mannan, that is to say, it contained no other carbohydrates and proteins.
Other Latest Articles
- Role of Salicylic Acid on Yield Improvement of ‘Elberta’ Peach (Prunus Persica L. Batsch) Tree
- Fungi Associated With the Powdery Mildew of Buxus Trees in Gorgan Landscape
- Evaluation of Antioxidative and Cytotoxic Properties of Ethanolic Extract of Salvia Officinalis on PC3 Human Prostate Cancer and Hela Cervical Cancer Cell Lines
- The Analysis of Things Which influence on Garlic Product’s Income in Zanjan Province
- The Effect of Supplemental Irrigation and Cultivars on Population of Safflower Fly (Acanthiophilus Helianthi Rossi.)
Last modified: 2019-07-05 03:06:10