PARTIAL PURIFICATION AND IMMUNE-BIOCHEMICAL CHARACTERIZATION OF DOG SERUM IMMUNOGLOBULIN G
Journal: Exploratory Animal and Medical Research (Vol.3, No. 1)Publication Date: 2013-06-16
Authors : Manoj Kumar; Subhasis Batabyal; Shyamsundar Kesh; Saibal Chattopadhyay;
Page : 36-40
Keywords : ;
Abstract
In the present study Immunoglobulin G was purified from serum of dog by gel filtration chromatography on Sephacryl S-200. SDS- PAGE analysis of purified dog IgG showed major polypeptides of 66 kDa, 52.40 kDa and 20.72 kDa. The purified Immunoglobulin has been found to be immune-reactive by DID test and Western Blot analysis when treated against hyperimmune sera which was raised in rabbit.
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