PARTIAL PURIFICATION AND IMMUNE-BIOCHEMICAL CHARACTERIZATION OF ROYAL BENGAL TIGER (PANTHERA TIGRIS TIGRIS) SERUM IMMUNOGLOBULIN G
Journal: Exploratory Animal and Medical Research (Vol.3, No. 2)Publication Date: 2013-12-16
Authors : Ekantika Mandal; Subhasis Batabyal; S. Chattopadhyay; S. S. Kesh; Abhijit Barui;
Page : 131-135
Keywords : ;
Abstract
In the present study Immunoglobulin G was purified from serum of Royal Bengal Tiger by gel filtration chromatography on Sephacryl S-200. SDS-PAGE analysis showed the molecular weight of purified tiger IgG was 170.52 kDa. The purified Immunoglobulin has been found to be immunereactive by DID test and Western Blot analysis when treated against hyperimmune sera which was raised in rabbit.
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