The Vitamin D Receptor (VDR) Gene Polymorphisms Fok1 In Obese Pakistanis– a Preliminary Report
Journal: International Journal of Advanced Biological and Biomedical Research (Vol.2, No. 9)Publication Date: 2014-09-01
Authors : Muhammad Haris Lucky; Saeeda Baig;
Page : 2569-2574
Keywords : Vitamin D; Obesity; VDR; Fok1 Polymorphism PCR-RFLP;
Abstract
Objective: The role of vitamin D has soared to a pinnacle in recent years with functions affecting 229 human genes linked to cardiovascular, autoimmune, humoral, pulmonary and neurological diseases. Relationship between obesity and vitamin D has not as yet been fully established. The objective of the study was to determine the possible relationship between Vitamin D receptor (VDR) gene polymorphisms and the risk of obesity. Methods: Total 100 subjects (50 obese and 50 normal) were selected. Oral rinse samples were collected (between 18-45 years of age) after an informed consent. DNA was extracted and PCR was performed using VDR-Fok1 primers. A 256bp amplified products was visualized by Gel Doc Hero Lab software (Germany). Results: The results of VDR-Fok1 gene polymorphism showed that out of 50 obese subjects 40%were normal (FF=20), 54%were Heterozygous (Ff = 27) and 6% were mutated (ff= 3). Out of 50 non-obese subjects 84% were normal (FF= 42), 16% were Heterozygous (Ff= 8) and none were mutated (ff= 0%). The statistical analysis results between the ff genotype and obesity were not significant. The ff genotype of VDR may be associated with obesity. More studies with larger sample size are required to find a stronger correlation.
Other Latest Articles
- Trichoderma Species Associated with Medicinal Plants
- Comparative Frequency Evaluation of Bacterial Organisms Isolated from Clinical Samples of ICU Admitted Patients and Related Factors in Ardabil-Iran
- Growth Performance, Blood Metabolites, Antioxidant Stability and Carcass Characteristics of Broiler Chickens Fed Diets Containing Nettle (Urtica dioica. L) Powder or Essential Oil
- Fusarium Species Associated with Foxtail Millet (Setaria Italica) in Iran
- The Production Pattern of Aflatoxin G1, G2 , by HPLC
Last modified: 2019-07-05 05:06:27