Optimized Root Production During Micropropagation of New Iranian Apple Hybrid Rootstock (AZ X M9): Effects of Fe-EDDHA and Thiamine
Journal: International Journal of Advanced Biological and Biomedical Research (Vol.2, No. 10)Publication Date: 2014-10-01
Authors : M. Haghgou Tabalvandani; A. Yadollahi; D. Atashkar; M. Eftekhari;
Page : 2659-2662
Keywords : Iranian; Hybrid rootstock; In vitro culture; Shoot regeneration; Rooting;
Abstract
Objective: In vitro propagation of AZ x M9 new apple rootstock resulted of breeding program of vegetative apple rootstock in Iran was investigated. Methods: Nodal explants were inoculated in Murashige and SKoog (MS) medium supplemented with various concentrations of 6-benzylaminon purine (BAP) (0.4, 0.8 and 1.2 mg/l) alone or with naphthalene acetic acid (NAA) (0.1 mg/l). We examined the effect of different concentrations of iron sequestrene (Fe-EDDHA) and thiamin vitamin in ½ MS and Linsmaier and Skoog (LS) media supplemented with indol-3-butric acid (IBA) (1 mg/l) on rooting of attained plantlets. Results: According to our results, 0.4 BAP + 0.1 NAA treatment in MS medium was the best component which resulted in the highest proliferation rate (6.73). As well, we estimated the highest root number in ½ MS + 0.15 g/l Fe-EDDHA + 2.4 g/l thiamin.
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