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Bimolecular identification of M.ovinpeumoniae from nasal swab samples of sheep from various districts of Balochistan, Pakistan

Journal: International Journal of Advanced Biological and Biomedical Research (Vol.4, No. 1)

Publication Date:

Authors : ; ; ; ; ; ; ; ; ;

Page : 32-39

Keywords : M.ovinpeumoniae; Sheep; Biochemical; PCR; Balochistan;

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Abstract

Mycoplasma ovipneumoniae is an important respiratory pathogen of sheep across the world. In the present study nasal swab samples (n=778) were processed and 51isolates of M. ovipneumoniae were identified through biochemical tests and PCR. Difference in the isolation rate among districts (n=12), age groups (n=3), breads (n=6) was assessed. Similarly the isolate rate among healthy (n=513) and respiratory distressed (n=265) sheep were also compared. The results for the district-wise isolation of Mycoplasma ovipneumoniae in sheep showed non-significant variations among districts of Balochistan (2= 1.65, P=0.9990). Statistically significant difference (2 = 7.698, P< 0.02) in the isolation of Mycoplasma ovipneumoniae among various age groups was recorded, while highest isolation was seen in sheep of < 1 year.Statistically highly significant difference (2 = 16.6, P < 0.0050) in the isolation of Mycoplasma ovipneumoniae among various breeds of sheep in Balochistan was seen, whereas highest isolation rate (3.70%) was observed in Harnai breed for sheep Furthermore, statistically highly significant difference (Fisher's exact test P≤0.0001) in the isolation of Mycoplasma ovipneumoniae between healthy and respiratory distressed sheep breeds in Balochistan was found.

Last modified: 2019-07-05 05:33:48