Bioorganic investigation of encapsulated Cysteine derivative into polymeric nanocarrier
Journal: International Journal of Advanced Biological and Biomedical Research (Vol.7, No. 4)Publication Date: 2019-10-01
Authors : Ehab M. Ali; Ahmed Hamed;
Page : 354-364
Keywords : biocompatibility; Neuroprotective; Polymer; biopolymer;
Abstract
In this work, the copolymer-based synthesized Cysteine-loaded nanocarriers prepared by a routine protocol, coprecipitation method. It is the first report to investigate the neuroprotective potential and biocompatibility of Cysteine derivatives loaded into poly(ethylene glycol)-block-poly(ε−caprolactone) methyl ether (PEG-b-PCL). The average size of the polymeric/empty NCs was 89 nm and for polymeric/Synthesized derivative of Cysteine was 126 nm. The Drug Loading efficiency was 81%. The concentration of Polymeric NCs was 2.1 x 10 10 particles/ml and the zeta potential of polymeric/empty and polymeric/ Synthesized derivative of Cysteine NCs -5 mV and -11 mV respectively. Biological part of this work were investigated in the SH-SY5Y human neuroblastoma cell line using cell viability and toxicity assays. The concentration of polymeric NCs below 1 x 10 10 particles/ml described as a zero-point damageable for the cell line. Also the Synthesized derivative of Cysteine encapsulated into polymeric NCs have more neuroprotective effect compared to free Cysteine at lower concentration, and therefore, have a significant neuroprotective potential against Z-VAD-fmk and St-evoked SH-SY5Y cell damage.
Other Latest Articles
- Evaluation of Spent Engine Oil and other Natural Materials on the Emergence of Harvester Ants (Messor galla Forel)
- Effect of sub-effective dose of GABA agonists on attenuation of morphine tolerance in rats: Behavioral and electrophysiological studies
- The evaluation and comparison of thermo-physical, chemical and biological properties of palladium (II) complexes on binuclear diamine ligands with different anions using the DFT method
- Evaluation of Neem Dust Formulations for the Control of Sorghum Stemborers in the Semi-Arid Zone of Nigeria
- Effect of Feeding Different Levels of Luffa aegyptiaca Extracts on the Growth Performance of Broiler Chicken Fed Corn-Soya Meal Diet
Last modified: 2019-07-05 05:58:52