Analytical Approaches in Glycobiology

Glycobiology is a study of the structure, chemistry, biosynthesis, and biological functions of glycans and their derivatives. The high complexity of biomolecules necessitates a set of powerful analytical methodologies to reveal functionally critical structural features. Mass spectrometry (MS), with its different ionization techniques, mass analyzers, and detection strategies, has become the most important analytical method in glycomic and glycoproteomic investigations. In combination with MS, microscale separations are based on capillary chromatography and electrophoresis and carbohydrate microchemistry, In this article focuses on Analytical, methodological advances on disease biomarker research, immunology, developmental biology, and measurements of importance to biopharmaceuticals.