TOXICITY OF SILVER NANOPARTICLES LOADED WITH Pleurotus tuber-regium EXTRACT ON RATS

The aim of the work was to provide synthesis and characterization of silver nanoparticles loaded with P. tuber-regium extract and to assess their acute toxicity and haemotoxicity activity on rats. Acute toxicity of silver nanoparticles was studied by up-and-down-procedure and haemotoxicity was analyzed by differential red bload cell (RBC) count. UV-visible spectroscopy analysis provided peak at 463.9 nm. Scanning electron microscopy analysis showed nanoparticles of 60.8 nm to 94.9 nm in size and spherical in shape. X-ray diffraction analysis showed 125.7 nm average sizes of the silver nanoparticles. Dynamic light scattering analysis provided the average diameter 71.4 nm and zeta potential of –11.2 mV of the synthesized nanoparticles. Fourier transform infrared spectroscopy analysis showed major transmission peaks at 3295.6 cm–1 corresponds to O-H stretch for alcohol and phenol 1602.2 cm–1 corresponds to N-H and C=C stretch for primary amine and conjugated alkene. Acute toxicity test showed no mortality, gross behavioural changes and decreased body weight. Significant increase in total RBC (4.30 ± 0.05 х 106/μL), phenotypic coefficient of variation paked cell volume (PCV 26.46 ± 0.01%) and total white blood cell (WBC 8.12 ± 0.27 х 103/μL) was observed in 400 mg/kg extract dose treatment group compared to control group. Thus, synthesized silver nanoparticles loaded with mediated by P. tuber-regium aquous extrac had no acute toxic, haemotoxic effect but they showed dose dependent haematinic and immune modulation activity.