UHPLC-DAD-ESI-MS/MS Analysis of Gossypitrin Sample from Talipariti Elatum (Sw.) With Antioxidant and Neuroprotective Effects

A sample of gossypitrin isolated from the ethanolic extracts of the petals of Talipariti elatum (Sw.) were scrutinized using an UHPLC/DAD/MS Thermo scientific Dionex Ultimate 3000 RS coupled to a Thermo scientific LTQ XL mass spectrometer. In addition, about six different chemical compounds were tentatively identified in this specie based on chromatography retention time (Rt), UV and MS/MS spectra and compared with those of isolated authentic compound and literature data. The flavonoids identified after UV and MS analyses were gossypitrin, isoquercitrin, hyperoside, quercetin-O-sambubioside, and possibly for the first time, two isomeric forms of kaempferol: 8-methoxy-kaempferol-3-O-glucose and 8-methoxy-kaempferol-3-O-galactose.