The Use of Digital Imaging Techniques for Better Understanding of Cellular Reactivity| Biomed Grid

Using ordinary techniques in viewing microscopic activities is limited by being qualitative in nature. Looking for more advanced understanding of image properties suffers from subjective views. Looking for more professional imaging analysis is available and not affordable in all settings. From our experience in immunohistochemistry, we used to localize biomarkers using indirect immunoproxidase techniques, but analyzing sections was a problematic and mainly beneficial for diagnosis since the result is mainly interpreted as positive or negative. The research needs in histopathology require more data for statistical analysis through examining hypotheses. To overcome these difficulties, we used the imaging analysis tools exist in adobe Photoshop software. The idea depends in calculating the total pixels per image, then an option to select a color of the image to compute its distribution as pixels. The obtained number is divided by the total pixels in slide. The resulting number is recorded. The same process is repeated for all sections that represent study groups. Using this technique leads to generate digital data that can be statistically analyzed and to compare the differences in groups and to determine the significance in occurring of cellular phenomena. Finally, we have used this technique for more than 10 years and published important articles in various journals