ANTI-BACTERIAL AND ANTI-INFLAMMATORY PROPERTY EVALUATION OF PARAMERIA LAEVIGATA (LUPIIT) FOR THE FORMULATION OF AN OINTMENT

This study evaluated the antibacterial and anti-inflammatory property of Parameria laevigata in the form of topical ointment. Chloroform and ethanolic solvents were used in the plant stem extraction. The extracts were subjected to phytochemical testing in which both extracts were positive with steroids and phenolic compounds. The antibacterial property was evaluated using paper disc diffusion method. The ethanolic and chloroform extracts with different concentration 2.5mg/ml; 5mg/ml; and 7.5mg/ml were evaluated against Escherichia coli, Staphylococcus aureus and Bacillus subtilis. The study showed that ethanolic crude extract of lupiit with the different concentrations have negative results while the chloroform extract was found to be partially active (10-13mm) on Staphylococcus aureus but not active to E. coli and B. subtilis. The chloroform crude extract was used in preparing the topical ointment. The anti-inflammatory activity was studied using albino rats through wound excision. Different concentrations were made 100 mg/kg BW of rat; 300 mg/kg BW of rat and 500 mg/kg BW of rat. The positive control used in the study was Betamethasone Mupirocin (Foskina-B) while the negative was distilled water. Based from the result of the study, all ointments made with different concentrations can be comparable to positive control. There was no significance difference in time duration among the different concentrations of ointment and the positive control. The best formulation was the 500mg ointment and was made as the final product. Therefore, the study showed that Parameria laevigata can be an antibacterial and anti-inflammatory ointment and it can be made available in the market.