Influence of Exogenous Factors on the Maturation Levels of Spermatozoa Chromatin in Sub/Infertile Men Treated with Nutritional Supplement PAPA®

Changes in the hereditary information during the nucleus maturation of the sperm cells can occur under the influence of exogenous or endogenous factors. Exogenous factors could be linked with unfavorable working environment or bad habits. In the recent years nutritional supplements have been used for male infertility treatment. In addition, they can improve the morpho-physiological status of the sperm. The present study aims to investigate the effect of nutritional supplement PAPA on the spermatozoa chromatin maturity level by using an Aniline Blue staining test (aniline blue staining - AB) in sub/infertile men exposed to exogenous factors.. A group of men (n = 88) was examined after their written agreement and a completed questionnaire. The tested group with established diagnoses of аsthenoteratozoospermia, oligoasthenoteratozoospermia, astenoterato- zoospermia with hypovolemia, oligoasthenoteratozoospermia with hypovolemia took orally PAPA® supplement for three months. All patients were tested for DNA damage by an AB test twice before and after the treatment. The patients in the tested group were divided based on the type of harmful work environment (I-IV) and harmful habits (smoking I-III). The results showed that after the treatment with a nutritional supplement PAPA®, the levels of condensation or maturity of sperm chromatin increased by 16.11%. In addition, connections between the effect of treatment and the conditions of the harmful working environment, as well as the amount of received tobacco by the smokers were found. A better effect of the treatment in the tested group with the absence of harmful working environment and non-smokers was found. Therefore, the lowest treatment efficacy was found in the patients who are chronically exposed to occupationally harmful environment, associated with increased body and scrotal temperature (seated workers in factories, ect., drivers, welders, bakers, founders, heavy physical labour workers). On the other hand, a negative connection between the smoked cigarettes per day and the effect of the food supplement in the tested group of men with harmful habits, such as smoking was established.