VALIDATION OF NEW LIQUID CHROMATOGRAPHIC METHOD FOR NATURALLY ISOLATED QUERCETIN AND ITS COMMERCIAL APPLICATION TO AYURVEDIC FORMULATION

Quercetin was isolated from Aerva lanata (L) and investigated for method development. A simple, accu- rate, reproducible method was developed using RP-HPLC. Commercial formulation of Quercetin 250mg capsules (Quercetin Complex-Solgar, USA) was used followed by an Ayurvedic proprietary medicine for the study. The chromatographic separation was performed on Shiseido Capcell Pak C 18 column (250 X 4.6 mm, 5 μm), mobile phase developed was 0.3% formic acid:acetonitrile:methanol (40:20:40) at the flow rate of 10μL/min at 22 0 C. The detector used was PDA with the detection wavelength of 370 nm. R T was 4.9mn with LOD-0.816 μg/ml and LOQ-2.473 μg/ml. The correlation coefficient was 0.992%. The linearity range was observed in the concentration range of 2-20 μg/mL and the recovery was found to be 99.91 to 102.65 %. Finally, the method applied to KACHCHNAR Ayurvedic formulation justified with characteristic peak for identifying quercetin in it.