Distribution and antibiotic pattern of non fermenting gram negative bacilli isolation in a tertiary care hospital

Introduction: Non Fermenting Gram Negative Bacilli (NFGNB) are group of organisms commonly present in hospital environment spreading through fomites. They are said to be isolated in 15% of all the isolates in infection. Owing to increase in drug resistance, there is a growing need to understand the pattern of presentation. This study was done to evaluate the distribution and pattern of presentation of NFGNB in isolates in hospital setting. Materials and Methods: This cross sectional study was carried out on 415 specimens analyzed in the laboratory of a tertiary care hospital for a period of one year and five months. All the clinical samples received were processed under standard protocol of inoculation and incubation. The blood culture bottles were placed in Bac T/ Alert 3 D and the positive culture bottle were processed by Grams stain and in routine bacteriological media for inoculation and incubated. The Non fermenting Gram negative organisms isolated were subjected for identification and antibiotic susceptibility profile by VITEK 2 and manual methods. Results: Majority of the isolates was identified as Pseudomonas spp (67.5%) followed by Acinetobacter spp (26.2%). Maximum specimens examined were urine samples (34.9%) followed by pus (25.3%). Overall, Amikacin, Ceftazidine and Cefipime were found to be the most sensitive antibiotic for NFGNB. Conclusion: There is a growing need to judicious use of antibiotics in NFGNB infections in order to prevent further antibiotic resistance thereby reducing health care costs and challenges in infectious disease management.