Pre-clinical evaluation of hepatoprotective activity of phytol in wistar albino rats

Introduction: Phytol, a phytoconstituent has been attributed for its hepatoprotective effect compared to Silymarin and hence present study. Aim: To study hepatoprotective activity of phytoconstituent Phytol in Ethanol induced experimental liver injury in rats. Materials and Methods: Wistar rats (male and female equal number) were divided into 5 groups consisting of 6 animals per group. The animals were pre-treated twice daily with 100 mg/kg and 200 mg/kg of Phytol which served as test group and Silymarin 100mg/kg orally which served as standard 1 hour before ethanol administration. All the animals except normal group animals were received ethanol (3.76 g/kg) twice daily for a period of 25 days. On 26th day, the animals were sacrificed to collect the sample to analyse levels of serum glutamic pyruvic transaminase (SGPT), alkaline phosphatase (ALP), serum glutamic oxaloacetic transaminase (SGOT), , cholesterol, triglyceride (TG), bilirubin, total protein (TP), super oxide dismutase (SOD), catalase (CAT) and glutathione (GSH). Phytol statistically reduced increased levels of SGPT, SGOT, ALP, TG, cholesterol and bilirubin in diseased animals. Phytol statistically increased reduced levels of TP, SOD, CAT, GSH in diseased animals. It was concluded that the phytoconstituent phytol have hepatoprotective activity. Statistical analysis. Statistical Analysis: It was done using Graph Pad Prism version 4 software (Graph Pad Inc., USA). ANOVA followed by Bonferroni's Multiple Comparison test was applied. Data presented as MEAN±SEM. Confidence level was taken at 95%. Results: The present study showed both low (100 mg/kg) and high (200 mg/kg) doses of phytol showed statistically significant (<0> Conclusion: Phytol, a phytoconstituent can be used as hepatoprotective agent as an alternate to Silymarin.