Prevalence of uropathogens in reproductive age group females and their antibiotic resistance pattern

Introduction: Urinary Tract Infection (UTI) remains the most common bacterial infection in human population. The prevalence of UTI is much higher in females as compared to males. The present study was planned with the aim to find out prevalence of uropathogens and their antibiotic resistance pattern in reproductive age group females. Materials and Methods: The present study was carried out in department of microbiology, Santosh Medical College and Hospital, Ghaziabad from Jan. 2018 to May 2018. Total 100 urine sample were processed for culture and sensitivity testing. Isolation of organism were done by inoculation of samples on Mac-Conkey Agar and Blood Agra media. After 24 hour of incubation at 370 C, each isolates were identified on the basis of morphology of colony in culture media, Gram stain, motility and biochemical reactions. Antibiotic sensitivity testing were carried out on Mueller Hinton Agar by Kirby Bauer method. Results: The prevalence of uropathogen was found 36%. The Gram-negative bacteria were most common isolates in comparison to Gram-positive bacteria. E. coli was the most common isolates followed by S. saprophyticus. Gram-negative organism were found more sensitive to Amikacin, Piperacillin Tazobactum, Ampicillin sulbactum, Cefoperazone sulbactum and Nitrofurantoin. Gram-positive bacteria were found more sensitive to Piperacillin Tazobactum, Tetracycline, Ampicillin sulbactum and Linezolid. E. coli were found more sensitive to Amikacin, Ampicillin sulbactum, Piperacillin Tazobactum and Cefoperazone sulbactum. Conclusion: Changing antimicrobial resistance pose challenge in treating urinary tract infections. Appropriate and judicious selection of antibiotic would limit the emerging drug resistant isolate in the future to treat this clinical condition successfully.