Comparative study of oxidative stress in type 2 diabetes mellitus with in vitro oxidative stress using ferrous ascorbate as pro-oxidant

Diabetes mellitus is a chronic endocrine disorder associated with a multitude of microvascular and macrovascular complications. The imbalance between the productions of free radicals plays a prominent role in the development of these complications. Different markers of oxidative stress on erythrocyte membrane like Malondialdehyde (MDA), Protein carbonyl, Protein thiols and protein content of the membrane show variations which can be estimated to know the effect of oxidative stress. Similar effect is seen on erythrocyte membranes when these are subjected to proxidants like Ascorbic acid with Iron or Copper in Vitro. In our study the effect of oxidative stress on RBC membrane in diabetes was estimated and the same was validated by subjecting Erythrocyte membranes of controls to oxidative stress in vitro by using Ascorbic acid with Iron. A case control comparative study was done with type 2 DM patient blood samples and normal controls at BMCH & RC, Chitradurga. The maximum number were of the age group of 41- 45 i.e. 32%. The mean FBS levels among cases and controls 197.50 mg/dl and 93.48 mg/dl respectively. There was similarity in the results between levels of protein content (4.56±0.19), protein carbonyl (1.20±0.08), protein thiol levels (1.42±0.10) and MDA (4.23±0.21) among diabetics in comparison to protein content (4.28±0.14), protein carbonyl (1.39±.0.097), protein thiols (1.04±0.05) and MDA (4.75±0.81) in controls treated with Iron Ascorbate a pro-oxidant mixture.