Efficiency of Probiotic in Immobilized Rats Through Involvement of the Antioxidant and Anti-inflammatory Systems

Background: Immobilization is known as type of stress that influences antioxidant status and inflammation factors. On the other hand, probiotics could significantly improve antioxidant status and inflammatory response. This study was thus conducted to evaluate the effects of probiotics in immobilized rats through involvement of the antioxidant and anti-inflammatory systems. Methods: A total number of 72 Albino Wistar rats were divided into 3 groups, including1) Normal control group without stress (Control), 2) Immobilized rats without additive (Immobilized), 3) Immobilized rats given with 104 CFU probiotic.day-1(Probiotic). Inflammatory and pro-inflammatory cytokines, corticostrone and serum antioxidants were evaluated in days 7 and 14. Results: Results showed that immobilization could significantly increase the serum concentrations of TNF-α, TGF-β corticostrone, MDA and NO and also decrease SOD, GPx and IL-10 in days 7 and 14, as immobilized rats were compared with control rats (P<0.05). However, administration of probiotic could significantly improve the above mentioned parameters (P<0.05). Conclusion: It could be concluded that the use of probiotics could alleviate adverse effects of stress. It could be advised to use of probiotics in form of dairy and other supplements to overcome on stress.