Bioinformatics Prediction of Interaction of Silver Nitrate and Nano Silver on Catalase and Nitrat Reductase

Silver nanoparticles (Ag-NPs) are one of the most commonly used nanoparticles, most particularly serving as an antimicrobial agent for medical applications. Silver or silver ions have long been used in many areas due to their strong antimicrobial activity against pathogenic microbes such as bacteria, yeast, fungi and algae.There have been two important enzymes of prokaryotic cells named Catalase and Nitrat reductase. Catalase is a common enzyme found in almost all living organisms which are exposed to oxygen. It catalyzes the decomposition of hydrogen peroxide to water and oxygen. It is an important enzyme in reproductive reactions. Nitrate reductase (Nar) is an important cellular enzyme which plays an essential role in cells. In this study we used Molegro virtual docker (MVD) and Metal Detector Predicts v2.0 software and also DiANNA 1.1 web server. The results obtained from docking showed us that the best pose which is derived from MolDock score for Catalase-peroxidase was -30.7299 with reranking score equal to -35. 1088 and the best pose derived from MolDock score for Catalaseperoxidase was -154.1820 with reranking score equal to -160.6844. The silver nanoparticles, Catalase-peroxidase and Nitrate reductase have interaction. In Nitrate reductase, silver nanoparticles make interaction with Cysteine 22, 19, 26, 54, 58, 61, 98, 101, 152 and 172 position in the enzyme. Obtained results showed that nanosilver and silver nitrate can be attached to Catalase-peroxidase and Nitrate reductase in prokaryotic cells leading to deactivate the enzymes.