Evaluating micro-propagation of Kashan damask rose, Yasooj aromatic rose and their hybrid

According to the value of damask rose and aromatic rose, crossing of Kashan damask rose × Yasooj aromatic rose has high importance in terms of its essential oil performance, rose water quality and its usage as a medicinal plant. With considering propagation problems of this plant, mass reproduction with tissue culture can help in a short time. Samples of crossed plant and their parents were collected from Research Institute of Forests and Rangelands. After pre-sterilization and sterilization, explants were established on the MS medium. When length of explants became around 2 cm, were transferred to MS medium with 3 different treatments for shooting and it was indicated that best shooting treatment for all 3 genotypes was MS medium with IBA (0.1 mg/l), 2ip (0.5 mg/l) and BA (0.5 mg/l). G22 genotype produced better shoots, so it was established on 8 different rooting treatments and finally MS media with IBA (2 mg/l) showed best result (100%). Then explants of (G22×G4) and G4 genotype also were established on the best rooting media and produced 28% and 0% roots, respectively. All the rooted plantlets were transferred to plastic pots containing cocopeat+ perlite+ vermicompost with the ratio of 2:1:0.5 and placed in the laboratory for compatibility. Totally it was concluded that between 3 genotype (crossed (G22×G4), damask rose as the paternal line (G4) and (G22) aromatic rose as the maternal line), G22 had highest results of micro-propagation, G4 had lowest results and crossed plant (G22×G4) was in the interstitial situation.