INVITRO OF FORMULATION AND EUGENOL RELEASE TEST USING VASELINE ALBUM BASE IN OINTMENT PREPARATION

Eugenol is an antibacterial agent that is able to inhibit or be bactericidal in Escherichia coli, Pseudomonas, Salmonella typhi and Staphylococcus aureus bacteria. The aimed of this research was to observe the characteristics of dosage form and release of eugenol ointment, and also to determine the effect of vaseline album concentration as a base and penetration enhancer of eugenol in the ointment preparation through membrane in vitro. Ointment was maked with vaseline album as base of ointmnet. Variation concentration of glyserin (82 g, 8 g, and 78 g) was added to eugenolointmnet. The evaluation included organoleptic, homogeneity, pH, viscosity, diffusion measurement, adhesion power, the contents of eugenol were analyzed by using UV-Vis spectrophotometry, and also eugenol release test from ointment base of each formula. Drug release test was carried out with Erweka Dissolution Tester with apparatus 5 paddle overdisk in phosphate buffer 7.4 ± 0.05, temperature 32°C, 200 rpm. The results showed that the differences in base vaseline albumin concentration influence ointment stability such as adhesion, dispersion, and eugenol dissolution profile. The increasing the concentration of vaseline album base, followed also by the increasing amount of eugenol released.