In Vitro Antiurolithiatic Activity of Kalyana Ksharam – A Herbo Mineral Formulation

PURPOSE To evaluate the in vitro antiurolithiatic activity of Kalyana Ksharam (KK). MATERIALS AND METHODS Inhibition of calcium oxalate (CaOx) crystal formation by KK was investigated by four different methods, namely, nucleation, aggregation, artificial urine and dissolution method. In nucleation, aggregation and artificial urine assay, the effectiveness of KK at different concentration (100, 200, 400, 600, 800 and 1000 µg/ml) on CaOx crystallization in vitro was evaluated, while in dissolution method the percentage of dissolution of CaOx crystals was investigated. RESULTS KK was effective in inhibiting CaOx crystals in dose-dependent manner and was comparable to that of cystone. CONCLUSIONS The present study suggests that KK has potential effect on in vitro inhibition of CaOx crystals.