PROTEOLITIC BACTERIA CULTURE OF ISOLATION RESULTS IN ORGAN LIVING OF FISHING GROUPER CULTURE RESULTS

The grouper is a sea water fish that is often cultivated. But in digesting feeds is often not optimal. To improve feed efficiency, one effort that can be done is to know the activity of proteolytic bacteria in milkfish digestion. The purpose of this study was to determine the presence and activity of proteolytic bacteria. The method used is the observation method with a purposive sampling technique. The abundance of bacteria in the digestive tract is calculated by the TPC (Total Plate Count) method. Based on the research results, it is known that there are proteolytic bacteria in the digestion group which are indicated by the presence of a clear zone around the bacterial colony that is grown on specific media (skim milk). There is also the activity of proteolytic bacteria in the digestion grouper by measuring the hydrolyzed clear zone with an average in the anterior part of the intestine 2.3 mm, middle 2.6 mm, posterior 2.65 mm. The results of this study indicate that the bacteria bacillus sp with sorbitol media, molasses and nutrient broth with a storage time of 40 days at 4 0C did not experience a decrease in bacterial density that was significantly different.