ROLE OF BIOTECHNOLOGY IN DISEASE MANAGEMENT OF OIL SEEDS (MUSTARD)

Indian mustard is one of the major rabi oilseed crop in India. Continuous improvement in rapeseed-mustard has resulted in nutritionally superior edible oil, and meal as an important source of protein in animal feed. The crop is affected by various biotic and abiotic stresses. There are many biotic stresses which affect the production of Mustard .Many attempts have been made by the scientists to improve Brassica using molecular markers like Amplified Fragment Length Polymorphism (AFLP), Simple Sequence Repeat (SSR), Single Nucleotide Polymorphism (SNP) based maps (Ramen et al., 2014) etc. Somaclonal variation as a tool for creating in vitro variability offers a unique opportunity for desirable attributes. Molecular markers such as Random Amplified Polymorphic DNA (RAPD), Restriction Fragment Length Polymorphism (RFLP), AFLP and SSR have been used for improving selection efficiency and selecting plant genotypes with the desired combinations of traits. Transgenic approaches have been followed to develop the transgenic for aphid resistance, male sterility, AB tolerance, herbicide resistance and drought tolerance. Bar, Barnase and Barstar based herbicide resistance and genetic male sterility have been used in the development of experimental hybrids