Assessment of Total Volatile Basic Nitrogen (TVB-N) and Microbial Contents of Iced Marine Fish Species

The total volatile basic nitrogen TVB N and microbial contents of iced species from the Arabian Gulf Shaeri Lethrinus miniatus and Hamour Epinephelus chlorostigma were investigated as indices of spoilage. On day 3 and till the end of storage period Hamour had higher pH values than Shaeri. The TVBN values in Hamour had risen from 15.9 mg 100g at zero time to 72.76 mg 100g at 5 days and then decreased to 64.3 mg 100g at 7 days. The TVBN had risen in shaeri from 10.5 mg 100g at zero time to 74.22 mg 100g at 5 days , Two fish marine species reached their maximum TVBN content on the 5th day of storage and were close to the rejection limit 30 mg 100 g by day 3 of storage. Bacteria grew rapidly between day 1 and 10. Hamour exceeded the maximum microbiological limit for fresh fish recommended by ICMSF , log 7.0 CFU g by day 7 of storage, while Shaeri did not reached that limit by day 7.day of storage. Initial total plate counts of marine fish species were 3.4 and 3.2 log 10 CFU g fish for Hamour and Shaeri respectively. Hamour and Shaeri psychrotrophic counts increased progressively with storage period. Initial psychrotrophic counts were 3.6 and 2.9 log 10 CFU g fish for Hamour and Shaeri, respectively these counts increased with the increase of storage time to reach a maximum values of 5.9 and 6.3 log 10 CFU g fish respectively at the end of storage. Shaeri had high coliform counts both initially and at the end of storage period than Hamour. proteolytic counts of all fish species increased with extended storage reaching their maximum count on the last day of storage period . Marine fish species had initially proteolytic counts of 2.3 and 1.4 log CFU g fish reaching a maximum value of 5.6 and 5.1 at the end of the storage period for Hamour and Shaeri respectively. The study showed that in both cases, the total volatile basic nitrogen TVB N and microbial contents had increased with increasing storage time. Ahmed Rashed Al-Najada "Assessment of Total Volatile Basic Nitrogen (TVB-N) and Microbial Contents of Iced Marine Fish Species" Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-4 | Issue-1 , December 2019, URL: https://www.ijtsrd.com/papers/ijtsrd29672.pdf Paper URL: https://www.ijtsrd.com/biological-science/microbiology/29672/assessment-of-total-volatile-basic-nitrogen-tvb-n-and-microbial-contents-of-iced-marine-fish-species/ahmed-rashed-al-najada