HPTLC Method Development and Validation for the Simultaneous Determination of Reserpine and Gallic acid in an Ayurvedic Formulation

A simple, precise, accurate, robust HPTLC method was developed and validated for simultaneous estimation of reserpine and gallic acid in an Ayurvedic formulation. The two markers were resolved using TLC plates pre-coated with silica gel 60F254 using the mobile phase Toluene: Acetone: Glacial acetic acid in the ratio 4:1:1 v/v with a saturation time of 10 minutes. The Rf values of gallic acid and reserpine were found to be 0.31 ± 0.2 and 0.54 ± 0.2, respectively at 268 nm. Linear response was observed in the concentration range of 20–80 ppm for reserpine and 150–210 ppm for gallic acid, with correlation coefficient (r2 ) of 0.995 and 0.997 for reserpine and gallic acid respectively. The developed method was applied for the quantitation of markers in a marketed formulation, Cardostab tablet. This method can also be used to evaluate other formulations containing reserpine and gallic acid as the constituents, thus conforming to the need of ensuring quality and safety of polyherbal formulations.