AMELIORATION IN INSULIN RESISTANCE AND Β-CELL FUNCTION BY DPP-4 INHIBITION POTENTIAL OF TRIGONELLA FOENUM SEED EXTRACT IN TYPE-2 DIABETIC RATS

The current study was aimed to evaluate amelioration in insulin resistance, β-cell function and pancreas protection by DPP-4 inhibition potential of Trigonella foenum (TF) seed extract in corticosteroid induced type-2 diabetic rats by in slilico, in vitro and in vivo assays. The corticosteroid induced diabetic status of animal model was considered at the HOMA indices, insulin and glucose levels. the in vitro assay of DPP-4 inhibition showed up to 60.4±2.8% and activity of DPP-4 in serum was observed to be 39.12±1.3% in TF seed extract treated groups. Furthermore, the FTIR spectra interpreted availability of potent functional groups in possessing bioactive compounds. Additionally, HPLC studies confirmed that gallic acid is the leading compound present in TF seed extract and shows significant binding energy obtained from -3.6 to -3.7 with DPP-4 residues LYS-71, ASN-74, GLU-91, THR-94, PHE-95, ILE-102, ASN-103, and ASP-104 via hydrophobic bonds. Significant changes were observed in HOMA indices, histopathology and others supportive parameters in treated groups. The study revealed promising results against insulin resistance, β-cell function and protective alterations in pancreas.