Analytical Method Development and Validation for Simultaneous Estimation of Paracetamol and Propyphenazone in their Combined Pharmaceutical Dosage Form by simultaneous Equation Method

UV Spectrophotometric method has been developed for simultaneous estimation of Paracetamol (PCM) and Propyphenazone (PP) in their combined pharmaceutical dosage form. This method utilizes methanol as a solvent and λmax of Paracetamol and Propyphenazone selected for analysis was found to be 249 nm and 274 nm respectively. Linearity was observed in the concentration range of 1-12 μg/ml for Paracetamol (r2=0.995) and 5-24 μg/ml for Propyphenazone (r2=0.997).The method was validated statistically and by recovery studies. The mean % recovery was 98.26-99.93% and 98.36-100.24% for Paracetamol and Propyphenazone respectively. Developed method was applied to pharmaceutical dosage form. The results were found to be within acceptance criteria according to ICH guideline. This method was simple, rapid, accurate and sensitive.